Difference between revisions of "Part:BBa K566000"

Revision as of 01:33, 28 September 2011

OL region from Lambda

OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).

Usage and Biology

OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda (1). Its binding sites maybe used to design of new regulatory networks or to mimic Lambda's natural behavior.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 OL region from Lambda phage. Allows biphasic switch ([https://parts.igem.org/Part:BBa_K566002 K566002]) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter ([https://parts.igem.org/Part:BBa_K566001 K566001]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda ([https://parts.igem.org/Part:BBa_K566000 1]). Its binding sites maybe used to design of new regulatory networks or to mimic Lambda's natural behavior.
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