Difference between revisions of "Part:BBa K566001:Design"

(→‎Source)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Analysis of illegal restriction sites was performed, but it does not have them originally.  
+
Original sequence does not have any illegal restriction sites.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 01:21, 28 September 2011

pRM promoter from Lambda


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence does not have any illegal restriction sites.

Source

Obtained by PCR directly from Lambda genomic DNA (GenBank accession number [http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 NC_001416]).

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Gene Dev 15:3013–3022.

2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.


Further Reading:

Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. J Bacteriol 189:298–304.