Difference between revisions of "Part:BBa K566010"
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<partinfo>BBa_K566010 short</partinfo> | <partinfo>BBa_K566010 short</partinfo> | ||
| − | Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis. | + | Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis. |
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Revision as of 00:55, 28 September 2011
PenI repressor optimized for E. coli (inverted sequence)
Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]