Difference between revisions of "Part:BBa K615004"

 
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<partinfo>BBa_K615004 short</partinfo>
 
<partinfo>BBa_K615004 short</partinfo>
  
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This is one of the two components necessary for a metabolic His3-URA3 selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase).
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This is one of the components necessary for a metabolic His3-URA3 one-hybrid selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase, and an E. coli strain with the genes HisB, PyrF, and rpoZ knocked out). The one-hybrid system, as developed by Meng et al (2005), ties zinc finger binding to cell survival
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 22:24, 27 September 2011

Zif268 binding site-His3-URA3 construct for one-hybrid selection

This is one of the components necessary for a metabolic His3-URA3 one-hybrid selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase, and an E. coli strain with the genes HisB, PyrF, and rpoZ knocked out). The one-hybrid system, as developed by Meng et al (2005), ties zinc finger binding to cell survival

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15
    Illegal BglII site found at 528
    Illegal BglII site found at 588
    Illegal BamHI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1508
    Illegal SapI.rc site found at 1355