Difference between revisions of "Part:BBa K615004:Design"

 
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<partinfo>BBa_K615004 short</partinfo>
 
<partinfo>BBa_K615004 short</partinfo>

Revision as of 21:54, 27 September 2011

Zif268 binding site-His3-URA3 construct for one-hybrid selection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15
    Illegal BglII site found at 528
    Illegal BglII site found at 588
    Illegal BamHI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1508
    Illegal SapI.rc site found at 1355


Design Notes

Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly.


Source

The sequence was synthesized.

References