Difference between revisions of "Part:BBa K566000"
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<partinfo>BBa_K566000 short</partinfo> | <partinfo>BBa_K566000 short</partinfo> | ||
| − | OL region from Lambda phage. Allows biphasic switch [https://parts.igem.org/Part:BBa_K566002 (K566002)] effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter(K566001). | + | OL region from Lambda phage. Allows biphasic switch [https://parts.igem.org/Part:BBa_K566002 (K566002)] effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter [https://parts.igem.org/Part:BBa_K566001 (K566001)]. |
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Revision as of 19:48, 27 September 2011
OL region from Lambda
OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]