Difference between revisions of "Part:BBa K678062"

Revision as of 18:48, 27 September 2011

pJEJAM14

pJEJAM14 is a plasmid that after digestion with NotI is intended to be transformed into Aspergillus nidulans and integrated into the genome by non-homologous end joining. The plasmid consists the strong constitutive gpdA promoter , monomeric red fluorescent protein (mRFP1), the trpC terminator, a pyrG marker cassette for selection, an ori for propagation in E. coli, and an ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM14, the figure is not drawn to scale.

When pJEJAM14 was integrated into A. nidulans expression of the gene encoding mRFP1, resulted in an even distribution of mRFP1 in the hyphae (Figure 2 and Figure 3). In figure 4 and figure 5 it be can see that the integration of the linearized pJEJAM14 must have impaired a gene or pathway of the transformant because its morphology and growth differed significantly from the [http://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi#Wild%20type wild-type].

DTU-Denmark-2 2011 Figure 2: differential interference contrast image of Aspergillus nidulans with pJEJAM14.

DTU-Denmark-2 2011 Figure 2: Fluorescence image of Aspergillus nidulans with pJEJAM14.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Front

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Back

Sequence and Features