Difference between revisions of "Part:BBa K678061"

Revision as of 18:48, 27 September 2011

pJEJAM13

pJEJAM13 is a plasmid that after digestion with NotI is intended to be transformed into Aspergillus nidulans and integrated into the genome by non-homologous end joining. The plasmid consists the strong constitutive gpdA promoter , GFP with the peroxisomal targeting sequence (PTS1) at the C-terminus, the trpC terminator, a pyrG marker cassette for selection, an ori for propagation in E. coli, and an ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.

Green fluorescence can be observed in the hyphae and in clear spots (Figure 3). This indicates that GFP is targeted to the peroxisomes, but further investigations are required to confirm this. In figure 4 and figure 5 it be can see that the integration of the linearized pJEJAM13 must have impaired a gene or pathway of the transformant because its morphology and growth differed significantly from the [http://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi#Wild%20type wild-type].

DTU-Denmark-2 2011 Figure 2: DIC image of Aspergillus nidulans with pJEJAM13.

DTU-Denmark-2 2011 Figure 3: 'Fluorescence image of Aspergillus nidulans with pJEJAM13. Green fluorescence can be observed in the hyphae and in clear spots.

DTU-Denmark-2 2011 Figure 4: Aspergillus nidulans Front

DTU-Denmark-2 2011 Figure 5: Aspergillus nidulans Back

Sequence and Features