Difference between revisions of "Part:BBa K676009:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K676009 short</partinfo> | <partinfo>BBa_K676009 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The ORF has a EcoR1 restriction site on the 6th bp position. | + | The ORF has a EcoR1 restriction site on the 6th bp position , thus a site directed mutagenesis has been carried out to remove the site. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 17:49, 27 September 2011
Gyrase B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 428
Illegal BglII site found at 1667 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 100
Illegal AgeI site found at 238
Illegal AgeI site found at 931
Illegal AgeI site found at 2047 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ORF has a EcoR1 restriction site on the 6th bp position , thus a site directed mutagenesis has been carried out to remove the site.
Source
The ORF comes from E.coli.