Difference between revisions of "Part:BBa K676008:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K676008 short</partinfo>
 
<partinfo>BBa_K676008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis is carried out to remove the restriction site.
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The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis has been carried out to remove the restriction site.
  
  

Latest revision as of 17:48, 27 September 2011

GyrA cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1819
    Illegal BglII site found at 2764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
    Illegal AgeI site found at 929
    Illegal AgeI site found at 986
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 2381
    Illegal AgeI site found at 2749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 269
    Illegal SapI.rc site found at 277
    Illegal SapI.rc site found at 1736


Design Notes

The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis has been carried out to remove the restriction site.


Source

Gyr A gene is obtained from E.coli genome.

References