Difference between revisions of "Part:BBa K676778"

 
 
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<partinfo>BBa_K676778 short</partinfo>
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<partinfo>BBa_K676014 short</partinfo>
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===Usage and Biology===
  
 
DNA gyrase is the only topoisomerase that introduces negative supercoiling into closed circular plasmid molecules. It is a heterotetramer consists of two GyrA and two GyrB subunits.
 
DNA gyrase is the only topoisomerase that introduces negative supercoiling into closed circular plasmid molecules. It is a heterotetramer consists of two GyrA and two GyrB subunits.
  
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This expression cassette is regulated by an IPTG inducible promoter and will be expressing both Gyr A and Gyr B subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. Gyr B subunit provides energy for the reaction in the presence of divalent cations such as magnesium cation.  
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This expression cassette is regulated by an IPTG inducible promoter and will be expressing both Gyr A and Gyr B subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. Gyr B subunit binds to ATP which will provide energy for the progress of catalytic reaction in the presence of divalent cations such as magnesium cation.  
  
 
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K676778 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K676014 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K676778 parameters</partinfo>
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<partinfo>BBa_K676014 parameters</partinfo>
 
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Latest revision as of 17:46, 27 September 2011

Extractery

Usage and Biology

DNA gyrase is the only topoisomerase that introduces negative supercoiling into closed circular plasmid molecules. It is a heterotetramer consists of two GyrA and two GyrB subunits.

This expression cassette is regulated by an IPTG inducible promoter and will be expressing both Gyr A and Gyr B subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. Gyr B subunit binds to ATP which will provide energy for the progress of catalytic reaction in the presence of divalent cations such as magnesium cation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1247
    Illegal NheI site found at 3558
    Illegal PstI site found at 1424
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 867
    Illegal BglII site found at 2329
    Illegal BglII site found at 4307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
    Illegal NgoMIV site found at 1033
    Illegal AgeI site found at 2975
    Illegal AgeI site found at 3045
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1520