Difference between revisions of "Part:BBa K634014"
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<partinfo>BBa_K634014 short</partinfo> | <partinfo>BBa_K634014 short</partinfo> | ||
This part was used as a component of an alkane sensor which utilized the transcription factor AlkS acting upon the promoter PalkB to express the red fluorescent protein tagRFP. | This part was used as a component of an alkane sensor which utilized the transcription factor AlkS acting upon the promoter PalkB to express the red fluorescent protein tagRFP. | ||
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| + | '''Note:''' This part is on the antisense strand, and can be considered reversed. Keep this in mind if cloning downstream of alkS. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 17:36, 27 September 2011
Reversed J23100-alkS
This part was used as a component of an alkane sensor which utilized the transcription factor AlkS acting upon the promoter PalkB to express the red fluorescent protein tagRFP.
Note: This part is on the antisense strand, and can be considered reversed. Keep this in mind if cloning downstream of alkS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 731
Illegal NgoMIV site found at 767
Illegal AgeI site found at 2046 - 1000COMPATIBLE WITH RFC[1000]