Difference between revisions of "Part:BBa K566011:Design"

 
 
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<partinfo>BBa_K566011 short</partinfo>
 
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Sequence was taken from BioBrick BBa_C0052
 
Sequence was taken from BioBrick BBa_C0052
  
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===References===
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==References==
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<b>1.</b> Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny  <i>Gene Dev</i> <b>15</b>:3013–3022.
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Latest revision as of 16:50, 27 September 2011

cI434 repressor from phage 434 optimized for E. coli cI (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 436
    Illegal AgeI site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and deleted.


Source

Sequence was taken from BioBrick BBa_C0052

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.