Difference between revisions of "Part:BBa K566015:Design"
 
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Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry <i>J Bacteriol</i> <b>186</b>:7899-7999.
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<b>1.</b>Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry <i>J Bacteriol</i> <b>186</b>:7899-7999.
  
 
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Latest revision as of 16:30, 27 September 2011

pRM-RBS-cI434


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
    Illegal AgeI site found at 558
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and deleted.


Source

Synthesized DNA.

References

1.Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry J Bacteriol 186:7899-7999.