Difference between revisions of "Part:BBa K566016:Design"

 
 
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===References===
 
===References===
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<p>1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.</p>
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<p>2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.
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Latest revision as of 16:24, 27 September 2011

OL-pTetMnt-cI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
    Illegal BglII site found at 168
    Illegal XhoI site found at 130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequences were optimized with preferential codons. Search for illegal sites was performed, sites were localized and deleted.


Source

Synthesized DNA.

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.

2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.