Difference between revisions of "Part:BBa K566019:Design"

 
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E. coli JT2 strain
 
E. coli JT2 strain
  
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===References===
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==References==
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<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
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<b>2.</b> Kuhlman TE, Cox EC (2010) Site-specific chromosomal integration of large synthetic constructs <i>Nucleic Acids Res</i> <b>38</b>:e92.
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Revision as of 16:06, 27 September 2011

E. coli MxRed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3043
    Illegal NheI site found at 3066
    Illegal NheI site found at 3999
    Illegal NheI site found at 4022
    Illegal PstI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 167
    Illegal NgoMIV site found at 3422
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Integration performed through protocol of Dr. Kuhlmann


Source

E. coli JT2 strain


References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.
2. Kuhlman TE, Cox EC (2010) Site-specific chromosomal integration of large synthetic constructs Nucleic Acids Res 38:e92.