Difference between revisions of "Part:BBa K566026:Design"
 
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==References==
 
==References==
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<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
 
<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
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Latest revision as of 15:48, 27 September 2011

Cph8 gene for red-photoreceptor (constitutive expression)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 167
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the project that this part was made, it was needed to use other Assambly Standard.


Source

CDS of Cph8 was extracted by PCR, promoter and terminator were Classic BioBricks.

References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.