Difference between revisions of "Part:BBa K678053"
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pJEJAM5 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, cyan fluorescent protein (CFP), the ''BGK'' polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'' and, ampR resistance cassette (Figure 1).
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pJEJAM5 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, cyan fluorescent protein (CFP), the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1).
  
 
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Expression of pJEJAM5 in the mammalian cell line U-2 OS results in a homogenous distribution of CFP in the cells (Figure 2 and Figure 3).
  
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[[Image:16_033.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
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[[Image:16_033.png|left|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM5 expressing CFP. The white bar has a length of 70μm.]]
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[[Image:16_blå.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
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[[Image:16_blue.png|right|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM5 expressing CFP. The white bar has a length of 50μm.]]
 
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Revision as of 15:28, 27 September 2011

pJEJAM5

pJEJAM5 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, cyan fluorescent protein (CFP), the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM5, the figure is not drawn to scale.



Expression of pJEJAM5 in the mammalian cell line U-2 OS results in a homogenous distribution of CFP in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM5 expressing CFP. The white bar has a length of 70μm.
DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM5 expressing CFP. The white bar has a length of 50μm.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
    Illegal NgoMIV site found at 3062
  • 1000
    COMPATIBLE WITH RFC[1000]