Difference between revisions of "Part:BBa K678052"
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<partinfo>BBa_K678052 short</partinfo> | <partinfo>BBa_K678052 short</partinfo> | ||
− | pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the | + | pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1). |
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+ | Expression of pJEJAM4 in the mammalian cell line U-2 OS results in a homogenous distribution of red fluorescent protein in the cells (Figure 2 and Figure 3). | ||
− | [[Image:5_rød-2.png|left| | + | [[Image:5_rød-2.png|left|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 70μm.]] |
− | [[Image:26.08.png|right| | + | [[Image:26.08.png|right|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 50μm.]] |
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Revision as of 15:22, 27 September 2011
pJEJAM4
pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).
Expression of pJEJAM4 in the mammalian cell line U-2 OS results in a homogenous distribution of red fluorescent protein in the cells (Figure 2 and Figure 3).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2207
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297
Illegal NgoMIV site found at 3062 - 1000COMPATIBLE WITH RFC[1000]