Difference between revisions of "Part:BBa K678060:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K678060 short</partinfo>
 
<partinfo>BBa_K678060 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
To be announced
+
This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.
 +
 
  
 +
[[Image:Overveiw_module_tails_2.png|left|450px|caption|<b>DTU-Denmark-2 2011</b>]]
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<br clear=all>
  
  
 
===Source===
 
===Source===
  
−
To be announced
+
See the individual biobricks
  
 
===References===
 
===References===

Latest revision as of 14:17, 27 September 2011

pJEJAM12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
    Illegal NotI site found at 5281
    Illegal NotI site found at 8107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3049
    Illegal BamHI site found at 4347
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
    Illegal NgoMIV site found at 3685
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4400
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3399
    Illegal SapI site found at 322


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References