Difference between revisions of "Part:BBa K678002"
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===Usage and Biology===
 
===Usage and Biology===
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We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below).
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We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (Figure 1).
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https://static.igem.org/mediawiki/igem.org/a/aa/Gfppts.png
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[[Image:Gfppts.png|left|400px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: eGFP targeted to the peroxisomes of the mammalian cell line U-2 OS.]]
  
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This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.  
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[[Image:Gfpptscfp.png|right|400px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: he mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.]]
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https://static.igem.org/mediawiki/igem.org/0/0b/Gfpptscfp.png
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Revision as of 10:08, 27 September 2011

eGFP-PTS1 for mammalian cells

Vector expressing eGFP targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1) from the cytomegalovirus (CMV) promoter with the BGH polyA tail and with a hygromycin resistance cassette.

Usage and Biology

We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (Figure 1).

DTU-Denmark-2 2011 Figure 1: eGFP targeted to the peroxisomes of the mammalian cell line U-2 OS.
DTU-Denmark-2 2011 Figure 2: he mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]