Difference between revisions of "Part:BBa K539461"

 
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<partinfo>BBa_K539461 short</partinfo>
 
<partinfo>BBa_K539461 short</partinfo>
  
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Built in Plac promoter([[Part:BBa_R0010]]), followed by vioD([[Part:BBa_K439413]]), 37’celcius regulator RBSs, tetR and terminator([[Part:BBa_K332033]]). It catalyze protodeoxyviolaceinic acid(PVA) into protoviolaceinic acid and inhibit Ptet contained circuit when the temperature hit 37’C or higher.
  
 
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Revision as of 09:50, 27 September 2011

promoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator

Built in Plac promoter(Part:BBa_R0010), followed by vioD(Part:BBa_K439413), 37’celcius regulator RBSs, tetR and terminator(Part:BBa_K332033). It catalyze protodeoxyviolaceinic acid(PVA) into protoviolaceinic acid and inhibit Ptet contained circuit when the temperature hit 37’C or higher.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1194
    Illegal SapI.rc site found at 1269