Difference between revisions of "Part:BBa K678002:Design"
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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below. | This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below. | ||
− | + | [[Image:Promotor_med_haler.png|left|250px|caption|<b>DTU-Denmark-2 2011</b>]] | |
+ | <br clear=all> | ||
− | This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid pJEJAM2 | + | This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678066 pJEJAM2] |
===Source=== | ===Source=== |
Revision as of 09:48, 27 September 2011
eGFP-PTS1 for mammalian cells
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2233
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
Illegal NgoMIV site found at 3088 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.
This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid pJEJAM2
Source
Source to be announced