Difference between revisions of "Part:BBa K678001:Design"
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| − | This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678058 pJEJAM10], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678059 pJEJAM11], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678067 pJEJAM19], and[https://parts.igem.org/wiki/index.php?title=Part:BBa_K678068 pJEJAM20] | + | This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678058 pJEJAM10], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678059 pJEJAM11], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678067 pJEJAM19], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678068 pJEJAM20] |
Latest revision as of 08:05, 27 September 2011
PalcA, inducible Aspergillus nidulans promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.
This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids pJEJAM10, pJEJAM11, pJEJAM19, and pJEJAM20
Source
Aspergillus nidulans genomic DNA
References
Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.: Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans. Biochem. J. (1985) 225: 449-454.