Difference between revisions of "Part:BBa K554007"
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HlyB is ...
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HlyB is part of the hemolysin secretion system ([http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_3:_Secretion_system Device 3, Protein Secretion System]), very important to export the proteins produced inside bacteria and that must act in targets outside (such as [https://parts.igem.org/Part:BBa_K554005 IL-12] and [https://parts.igem.org/Part:BBa_K554004 IL-10]). HlyB acts as a ATP-binding cassette, and recognizes the substrate via its secretion signal (like HlyA) and is responsible for the specificity of the secretion system process. This system is composed of 4 essential parts: the C-terminal signal sequence of alpha-hemolysin ([https://parts.igem.org/Part:BBa_K554002 HlyA], which will be linked to the export target protein), the two specific translocator proteins HlyB and [https://parts.igem.org/Part:BBa_K554008 HlyD] and the outer membrane protein [BBa_K554009 TolC].  
  
 
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===Usage and Biology===
 
  
 
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===Usage and Biology===

Revision as of 05:01, 27 September 2011

Hemolysin B - HlyB

HlyB is part of the hemolysin secretion system ([http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_3:_Secretion_system Device 3, Protein Secretion System]), very important to export the proteins produced inside bacteria and that must act in targets outside (such as IL-12 and IL-10). HlyB acts as a ATP-binding cassette, and recognizes the substrate via its secretion signal (like HlyA) and is responsible for the specificity of the secretion system process. This system is composed of 4 essential parts: the C-terminal signal sequence of alpha-hemolysin (HlyA, which will be linked to the export target protein), the two specific translocator proteins HlyB and HlyD and the outer membrane protein [BBa_K554009 TolC].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1301
    Illegal BglII site found at 1964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology