Difference between revisions of "Part:BBa K634007"

Revision as of 03:51, 27 September 2011

Double selection cassette (tagRFP-sacB-kanR)

When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:

Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)

Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.

Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 648
Illegal SapI.rc site found at 30

@@ Line 3: / Line 3: @@
 When placed 3' of the promoter portion of a 1- or 2-component sensor,  BBa_634007 can be used in the following ways:
 Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)
 Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.
 Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose