Difference between revisions of "Part:BBa K634007:Design"

 
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===Design Notes===
 
===Design Notes===
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We are still in the process of optimizing this device, the following considerations are undergoing testing:
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We are still in the process of optimizing this device, the following considerations are undergoing testing:<p>
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tagRFP was taken from an expression vector, did it lose 5' components essential for high expression?
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tagRFP was taken from an expression vector, did it lose 5' components essential for high expression?<p>
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Can we reduce the frequency of spontaneous loss of function mutations in sacB through protein fusion with kanR?
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Can we reduce the frequency of spontaneous loss of function mutations in sacB through protein fusion with kanR?<p>
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Is the kanR RBS too strong for the desired resolution of susceptibility? Can a weakened RBS increase the range of the selection?
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Is the kanR RBS too strong for the desired resolution of susceptibility? Can a weakened RBS increase the range of the selection?<p>
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Likewise, is the RFP of K322921 too weak? Are we not getting enough translation of sacB to kill the population?
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===Source===
 
===Source===

Revision as of 03:48, 27 September 2011

Double selection cassette (tagRFP-sacB-kanR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 648
    Illegal SapI.rc site found at 30


Design Notes

We are still in the process of optimizing this device, the following considerations are undergoing testing:

tagRFP was taken from an expression vector, did it lose 5' components essential for high expression?<p> Can we reduce the frequency of spontaneous loss of function mutations in sacB through protein fusion with kanR?<p> Is the kanR RBS too strong for the desired resolution of susceptibility? Can a weakened RBS increase the range of the selection?<p> Likewise, is the RFP of K322921 too weak? Are we not getting enough translation of sacB to kill the population?

Source

tagRFP came from Evrogen sacB and kanR came from the registry

===References===