Difference between revisions of "Part:BBa K615003"
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<partinfo>BBa_K615003 short</partinfo>
 
<partinfo>BBa_K615003 short</partinfo>
  
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This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell.  The omega subunit of RNA polymerase has been knocked out using lambda red to replace the gene with a zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit fusion protein.
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This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell.  The omega subunit of RNA polymerase has been knocked out using lambda red to replace the gene with a zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit fusion protein.  See below for a summary of features and techniques.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K615003 parameters</partinfo>
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[[Image: HARVtolcsummary.png]]
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Revision as of 21:57, 26 September 2011

E. coli strain for TolC one-hybrid selection system

This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell. The omega subunit of RNA polymerase has been knocked out using lambda red to replace the gene with a zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit fusion protein. See below for a summary of features and techniques.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 845
    Illegal BglII site found at 965
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 121
    Illegal SapI site found at 331


Functional Parameters

HARVtolcsummary.png