Difference between revisions of "Part:BBa K615003"

Revision as of 21:46, 26 September 2011

E. coli strain for TolC one-hybrid selection system

This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell. The omega subunit of RNA polymerase has been knocked out using lambda red to replace the gene with a zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit fusion protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 632
Illegal PstI site found at 952
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 632
Illegal PstI site found at 952
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 845
Illegal BglII site found at 965
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 632
Illegal PstI site found at 952
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal NgoMIV site found at 181
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 121
Illegal SapI site found at 331

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 __NOTOC__
 <partinfo>BBa_K615003 short</partinfo>
-This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell.
+This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell.  The omega subunit of RNA polymerase has been knocked out using lambda red to replace the gene with a zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit fusion protein.
 <!-- Add more about the biology of this part here