Difference between revisions of "Part:BBa K566006"

Revision as of 18:09, 26 September 2011

penI-pCpcG2-pPenI-mnt

Repression-based potential AND/multiple input gate. Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. This part is intended to be used as part of a logical AND gate (or multiple input gate) to control gene expression from pMnt. Only when green light is on and any other Mnt source is off, expression from pMnt is allowed.

This construction may be used with another one which represses mnt expression with red light and this way it may act as an and-gate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 992
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 912
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K566006 short</partinfo>
-Potential and gate.
+'''Repression-based potential AND/multiple input gate.''' Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. This part is intended to be used as part of a logical AND gate (or multiple input gate) to control gene expression from pMnt. Only when green light is on and any other Mnt source is off, expression from pMnt is allowed.
-Composed of pCpcG2 promoter which may be inducible by green light, this way it express penI and this represses mnt expression, allowing the transcription of any gene controlled under pMnt promoter.
 This construction may be used with another one which represses mnt expression with red light and this way it may act as an and-gate.