Difference between revisions of "Part:BBa K634007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | ''sacB'' appears to be something of a lightning rod for loss of function mutations. As can be seen below, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in ''sacB'' produce sucrose-insensitive mutants. | ||
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Revision as of 15:46, 26 September 2011
Double selection cassette (tagRFP-sacB-kanR)
When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways: Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence) Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin. Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 648
Illegal SapI.rc site found at 30