Difference between revisions of "Part:BBa K535008:Design"
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===Design Notes=== | ===Design Notes=== | ||
In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp. | In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 03:27, 26 September 2011
repC - ctRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 835
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 916
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 761
Illegal SapI site found at 955
Design Notes
In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp.
Source
Low copy number plasmid pRmeGR4a from Sinorizobium meliloti GR4.
References
1. Izquierdo, Javier et al. An antisense RNA plays a central role in the replication control of a repC plasmid. 2005. Plasmid 54, 259-277.
2. Rogottier-Gois, Lionel. Distribution of repC plasmid-replication sequences among plasmids an sisolatees of Rhizobium leguminosarum bv. viciae from field populations. 1998. Microbiology, 144, 771-780.