Difference between revisions of "Part:BBa K535008:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K535008 short</partinfo>
 
<partinfo>BBa_K535008 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp.
 
In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp.
−
 
  
  
 
===Source===
 
===Source===
  
−
Low copy number plasmid pRmeGR4a from Sinorizobium meliloti GR4.  
+
Low copy number plasmid pRmeGR4a from ''Sinorizobium meliloti'' GR4.  
  
 
===References===
 
===References===
 +
1. Izquierdo, Javier et al. An antisense RNA plays a central role in the replication control of a repC plasmid. 2005. Plasmid 54, 259-277.
 +
 +
2. Rogottier-Gois, Lionel. Distribution of repC plasmid-replication sequences among plasmids an sisolatees of Rhizobium leguminosarum bv. viciae from field populations. 1998. Microbiology, 144, 771-780.

Revision as of 03:27, 26 September 2011

repC - ctRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 835
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 761
    Illegal SapI site found at 955


Design Notes

In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp.


Source

Low copy number plasmid pRmeGR4a from Sinorizobium meliloti GR4.

References

1. Izquierdo, Javier et al. An antisense RNA plays a central role in the replication control of a repC plasmid. 2005. Plasmid 54, 259-277.

2. Rogottier-Gois, Lionel. Distribution of repC plasmid-replication sequences among plasmids an sisolatees of Rhizobium leguminosarum bv. viciae from field populations. 1998. Microbiology, 144, 771-780.