Difference between revisions of "Part:BBa K575044:Design"
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<partinfo>BBa_K575044 short</partinfo> | <partinfo>BBa_K575044 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP. | |
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===Source=== | ===Source=== | ||
| − | + | Other than the genomic promoter, the pieces of this construct are all existing registry parts. | |
===References=== | ===References=== | ||
Latest revision as of 01:41, 26 September 2011
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1562
Illegal NheI site found at 1585 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1941
Illegal AgeI site found at 2138 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1471
Design Notes
The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
Source
Other than the genomic promoter, the pieces of this construct are all existing registry parts.