Difference between revisions of "Part:BBa K575044"

 
 
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<partinfo>BBa_K575044 short</partinfo>
 
<partinfo>BBa_K575044 short</partinfo>
  
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Genomic LasP. producing GFP along with constiutivley producing LasR
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This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter from the ''Pseudomonas'' genome with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.
  
 
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Latest revision as of 01:40, 26 September 2011

Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter from the Pseudomonas genome with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1562
    Illegal NheI site found at 1585
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1941
    Illegal AgeI site found at 2138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1471