Difference between revisions of "Part:BBa K575043:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K575043 short</partinfo>
 
<partinfo>BBa_K575043 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
no
 
  
 +
The primers used to extract the promoter had the biobrick restriction sites to ensure we could make it compatible in an assembly such as this.
  
  
 
===Source===
 
===Source===
  
−
from genome and registry
+
The promoter is from the ''Pseudomonas Aeruginosa'' genome, the other parts are from the registry.
  
 
===References===
 
===References===

Latest revision as of 01:38, 26 September 2011

Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1194


Design Notes

The primers used to extract the promoter had the biobrick restriction sites to ensure we could make it compatible in an assembly such as this.


Source

The promoter is from the Pseudomonas Aeruginosa genome, the other parts are from the registry.

References