Difference between revisions of "Part:BBa K575037:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K575037 short</partinfo>
 
<partinfo>BBa_K575037 short</partinfo>
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===Design Notes===
 
===Design Notes===
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put the constitutive promoter after the production of RhlR
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The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
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===Source===
 
===Source===
  
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from registry parts
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The pieces of this construct are all existing registry parts.
  
  
 
===References===
 
===References===

Latest revision as of 01:28, 26 September 2011

RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0034) + RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 814
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1583
    Illegal BsaI.rc site found at 723


Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.


References