Difference between revisions of "Part:BBa K575037"
 
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__NOTOC__
 
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This construct is designed to report the presence of PAI2, part of the ''Pseudomonas'' quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
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[[Image:rhlp-34-gfp_cp-34-rhlr.jpg]]
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<partinfo>BBa_K575037 short</partinfo>
 
<partinfo>BBa_K575037 short</partinfo>
  
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Promoter activated by RhlR/C4-HSL complex. Activation leads to GFP expression.
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Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.
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Continuous expression of RhlR.
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[[Image:rhlp-34-gfp_cp-34-rhlr.jpg]]
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:28, 26 September 2011

This construct is designed to report the presence of PAI2, part of the Pseudomonas quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. Rhlp-34-gfp cp-34-rhlr.jpg


RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0034) + RhlR

Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 814
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1583
    Illegal BsaI.rc site found at 723