Difference between revisions of "Part:BBa K575033"
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+ | This construct is designed to report the presence of PAI2, part of the ''Pseudomonas'' quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. | ||
+ | [[Image:rhlp-30-gfp_cp-34-rhlr.jpg]] | ||
+ | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K575033 short</partinfo> | <partinfo>BBa_K575033 short</partinfo> | ||
− | + | Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter. | |
− | + | ||
− | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 01:19, 26 September 2011
This construct is designed to report the presence of PAI2, part of the Pseudomonas quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0034) + RhlR
Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 817
Illegal NheI site found at 840 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1111
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1586
Illegal BsaI.rc site found at 726