Difference between revisions of "Part:BBa K575028:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K575028 short</partinfo> | <partinfo>BBa_K575028 short</partinfo> | ||
<partinfo>BBa_K575028 SequenceAndFeatures</partinfo> | <partinfo>BBa_K575028 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP. | |
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===Source=== | ===Source=== | ||
| − | + | The pieces of this construct are all existing registry parts. | |
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===References=== | ===References=== | ||
Latest revision as of 01:05, 26 September 2011
LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
Illegal NheI site found at 918
Illegal NheI site found at 941 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1297
Illegal AgeI site found at 1494 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 827
Design Notes
The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
Source
The pieces of this construct are all existing registry parts.