Difference between revisions of "Part:BBa K575028"

Revision as of 01:03, 26 September 2011

When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 58
Illegal NheI site found at 918
Illegal NheI site found at 941
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1297
Illegal AgeI site found at 1494
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 827

LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

@@ Line 1: / Line 1: @@
-<partinfo>BBa_K575028 short</partinfo>
-Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 15: / Line 10: @@
 <partinfo>BBa_K575028 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K575028 short</partinfo>
+Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K575028 parameters</partinfo>
 <!-- -->