Difference between revisions of "Part:BBa K575027"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K575027 short</partinfo> | <partinfo>BBa_K575027 short</partinfo> | ||
| − | + | This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1. | |
| − | + | ||
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 00:33, 26 September 2011
LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0034) + LasR
This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
Illegal NheI site found at 907
Illegal NheI site found at 930 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1283
Illegal AgeI site found at 741
Illegal AgeI site found at 853
Illegal AgeI site found at 1480 - 1000COMPATIBLE WITH RFC[1000]