Difference between revisions of "Part:BBa K575024:Design"

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<partinfo>BBa_K575024 SequenceAndFeatures</partinfo>
 
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===Design Notes===
 
===Design Notes===

Latest revision as of 00:26, 26 September 2011

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830

Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.

References