Difference between revisions of "Part:BBa K575025"

 
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<partinfo>BBa_K575025 short</partinfo>
 
<partinfo>BBa_K575025 short</partinfo>
  
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Continuous expression of LasR combined with receptor
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This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and GFP. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.
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Revision as of 00:25, 26 September 2011

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0034) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and GFP. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
    Illegal AgeI site found at 1494
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830