Difference between revisions of "Part:BBa K642004"
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<partinfo>BBa_K642004 short</partinfo> | <partinfo>BBa_K642004 short</partinfo> | ||
| − | This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of ATc (1). This repressor is tagged with yeast codon optimized BFP as well as a VP16 activation domain. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators. | + | This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of ''E.coli''. It will bind with high specificity to the ''tetO'' operator and is inhibited in the presence of ATc (1). This repressor is tagged with yeast codon optimized BFP as well as a VP16 activation domain. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators. |
===References=== | ===References=== | ||
Revision as of 23:57, 25 September 2011
TetR repressor tagged with yBFP and a VP16 activation domain
This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of ATc (1). This repressor is tagged with yeast codon optimized BFP as well as a VP16 activation domain. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
References
(1) Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69.
(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
(3) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." Proc Natl Acad Sci U S A 97(14):7963-8.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]