Difference between revisions of "Part:BBa K575046:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K575046 short</partinfo>
 
<partinfo>BBa_K575046 short</partinfo>
  
 
<partinfo>BBa_K575046 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K575046 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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no
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The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
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+
−
 
+
  
 
===Source===
 
===Source===
  
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from genome and registry
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Except for the genomic promoter, the pieces of this construct are all existing registry parts.
  
 
===References===
 
===References===

Latest revision as of 21:36, 25 September 2011

Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1282
    Illegal NheI site found at 1305
    Illegal NotI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2051
    Illegal BsaI.rc site found at 1191

Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

Except for the genomic promoter, the pieces of this construct are all existing registry parts.

References