Difference between revisions of "Part:BBa K575024:Design"
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===Source=== | ===Source=== | ||
| − | + | The pieces of this construct are all existing registry parts. | |
===References=== | ===References=== | ||
Revision as of 21:21, 25 September 2011
LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
Illegal NheI site found at 921
Illegal NheI site found at 944 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1300
Illegal AgeI site found at 1497 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 830
Design Notes
The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
Source
The pieces of this construct are all existing registry parts.