Difference between revisions of "Part:BBa K642005"
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<partinfo>BBa_K642005 short</partinfo>
 
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LacI repressor tagged upstream with yeast codon optimized blue fluorescent protein and a VP16 activation domain.  
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This is the LacI repressor from ''E.coli'' tagged with yeast codon optimized BFP and a VP16 activation domain. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
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===References===
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(1) Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.
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(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
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(3)
  
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===Usage and Biology===
 
  
 
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Revision as of 21:11, 25 September 2011

LacI repressor tagged with yBFP and a VP16 activation domain

This is the LacI repressor from E.coli tagged with yeast codon optimized BFP and a VP16 activation domain. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.

(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

(3)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1084
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]