Difference between revisions of "Part:BBa K642004"
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<partinfo>BBa_K642004 short</partinfo>
 
<partinfo>BBa_K642004 short</partinfo>
  
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This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. The repressor is tagged with yeast codon optimized BFP synthesized by the uOttawa Team.  
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This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of ATc (1). This repressor is tagged with yeast codon optimized BFP as well as a VP 16 activation domain. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.  
  
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===References===
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===Usage and Biology===
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(1) Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69.
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(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
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(3)
  
 
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Revision as of 21:07, 25 September 2011

TetR repressor tagged with yBFP and a VP16 activation domain

This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of ATc (1). This repressor is tagged with yeast codon optimized BFP as well as a VP 16 activation domain. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69.

(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

(3)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]