Difference between revisions of "Part:BBa K642003"

Revision as of 21:01, 25 September 2011

LexA repressor tagged with yBFP

This is the lexA repressor that is tagged with yeast codon optimized BFP. LexA will bind to an operator already present in the registry: BBa_K105022. This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 607
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K642003 short</partinfo>
-This is the lexA repressor that is tagged with yeast codon optimized BFP. LexA will bind to an operator already in the registry: BBa_K105022. This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
+This is the lexA repressor that is tagged with yeast codon optimized BFP. LexA will bind to an operator already present in the registry: BBa_K105022. This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
 ===References===